Generator

Part:BBa_K2278002:Design

Designed by: Paul ZANONI   Group: iGEM17_INSA-UPS_France   (2017-10-08)


Vibrio cholerae CAI-1 (quorum sensing inducer) generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 619
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Terminator : BBa_B1006* is mutated at the 35th pair (initial A->T) due to IDT complexity requirements for gBlocks synthesis. The effect of the mutation was not investigated.


Source

This enzyme cames from Vibrio cholerae DNA genomic sequence.

References